51猎奇

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Madhusudan Dey

  • Member, Milwaukee Institute for Drug Discovery (MIDD)

Professor of Biological Science

Education

  • PhD, Life Sciences, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, 1994 - 2000
  • MSc, Biotechnology, School of Biotechnology, Madurai Kamaraj University, Tamil Nadu, India, 1992-1994

Research Interests

The Dey lab is interested in understanding the fundamental mechanisms that regulate the initiation of protein synthesis (translation) and that regulate the protein folding inside the endoplasmic reticulum (ER).

Translation is a process in which messenger RNA is decoded into a polypeptide chain. This process requires many trans-acting factors (e.g., eIF1, eIF2, eIF2B, eIF3, eIF4, eEF1, etc.) that interact with the ribosome, messenger RNA and transfer RNA. This process is controlled by many protein kinases, including PKR, PERK and GCN2. The Dey lab studies how PKR and PERK specifically phosphorylates eIF2 and how phosphorylated eIF2 inhibits the initiation of protein synthesis.

Almost 30% of nascent proteins co-translationally migrate to the ER, where they fold and mature into biologically active forms. Under patho-physiological conditions, the nascent proteins are unable to fold properly, resulting in accumulation of unfolded/mis-folded proteins inside the ER 鈥� a state termed 鈥淓R stress鈥�. The ER stress activates cellular responses known as the unfolded protein response (UPR). The UPR involves three major sensors Ire1, PERK and ATF6 in mammalian species. In model organism yeast, only Ire1 senses and signals the UPR. Ire1 initiates the UPR signals by processing the translationally repressed听HAC1听mRNA in yeast cells or听XBP1听mRNA in human cells. The Dey lab studies how听HAC1听mRNA is translationally repressed and how the ER stress de-represses听HAC1听mRNA translation.

Recently, the Dey lab has discovered that kinase Kin1 and its isoform Kin2 (mammalian homolog of microtubule affinity regulating kinase, MARK) play a novel regulatory role in the yeast UPR, in addition to their canonical role in cellular exocytosis. This discovery leads them to characterize the Kin-signaling pathway in the yeast UPR.

Selected Publications

George J, Li Y, Kadamberi IP, Parashar D, Tsaih SW, Gupta P, Geethadevi A, Chen C, Ghosh C, Sun Y, Mittal S, Ramchandran R, Rui H, Lopez-Berestein G, Rodriguez-Aguayo C, Leone G, Rader JS, Sood AK, Dey M, Pradeep S, Chaluvally-Raghavan P. Cell Rep. (2023), 42 (3), 112228.

Uppala, Jagadeesh K., Sathe, Leena, and Dey, Madhusudan.听听Journal of Biological Chemistry. (2022), 298 (3), 101648.

Dey, Madhusudan, and Uppala, Jagadeesh K. 听Bio-Protocol. (2022), 20 (4), e4331.
George, Jasmine, Dey, Madhusudan, and Chaluvally-Raghavan, Pradeep.听听Cell Reports听Nov 2;37(5. (2021): 109934.
Ghosh, Chandrima, Uppala, Jagadeesh K., and Dey, Madhusudan. Science Signaling. (2021), 25 (14), eaaz4401.
Dey, Madhusudan, and Uppala, Jagadeesh.听听Molecular and Cellular Biology. (2021), 41 (7), e0066220.
Sathe, Leena, Bolinger, C, Mannan, MA, Dever, TE, and Dey, Madhusudan. 听J Biol Chem听290. Ed. Dey, Madhusudan. (2015): 21821-32.
Anshu, A, Mannan, MA, Chakrabarty, A, Chakrabarti, S, and Dey, Madhusudan.Mol Cell Biol.听35. Ed. D, Madhusudan. (2015): 199-210.
Dey, Madhusudan. 鈥淎ctivation of Protein Kinase PKR Requires Dimerization-induced cis-Phosphorylation within the Activation Loop.鈥澨�Journal Biological Chemistry听289. (2014): 5747-5757.
Mannan, M. Amin-ul, Shadrick, William R., Biener, Gabriel, Anshu, Ashish, Raicu, Valerica, Frick, David N., and Dey, Madhusudan.听听J. Mol. Biol.听425. (2013): 2083-99.
Dey, Madhusudan. 听Proc. Natl. Acad. Sci. USA听108.11 (2011): 4312-21.